Wnt5a Does Not Support Hematopoiesis in Stroma-Free, Serum-Free Cultures

Wnt5a Does Not Support Hematopoiesis in Stroma-Free, Serum-Free Cultures

Previously we reported that Wnt5a is highly expressed in the murine urogenital ridge-derived UG26-1B6 cells but not embryonic liver-derived EL08-1D2 cells. Mouse long-term repopulating hematopoietic stem cells (LTR-HSC) were maintained in non-contact UG26-1B6 cultures but not EL08-1D2 non-contact cultures, unless Wnt5a was also added to the cultures, suggesting a role for Wnt5a in the in vitro ...

Fibrinogen synthesis in serum-free hepatocyte cultures: stimulation by glucocorticoids.

Fibrinogen synthesis was investigated in cultures of chicken embryo hepatocytes initiated and maintained in chemically defined, serum-free medium. 11-Hydroxy glucocorticoids caused a 3-fold stimulation of fibrinogen synthesis. Half-maximal stimulation was achieved with 1 nM corticosterone or hydrocortisone, as compared with only 0.1 nM dexamethasone. Increased fibrinogen production in the prese...

Brown Representability Does Not Come for Free

We exhibit a triangulated category T having both products and coproducts and a triangulated subcategory S ⊂ T which is both localizing and colocalizing, and for which neither a Bousfield localization nor a colocalization exists. It follows that neither the category S nor its dual satisfy Brown representability. Our example involves an abelian category whose derived category does not have small ...

Abnormal serum free light chain ratio does not always indicate monoclonal gammopathy.

High correlation between salivary cortisol and free serum cortisol measurements does not mean good agreement.

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